| International Session（Symposium）3(JSGS・JSGE・JSH・JSGCS) |
| Fri. November 22nd 14:00 - 16:30 Room 2: Kobe International Exhibition Hall No.2 Building Hall (South) |
| Application of Helicobacter pylori (HP)-related DNA methylation in the gastric tissue | |||
| Tomomitsu Tahara1, Kazuichi Okazaki1, Naoki Ohmiya2 | |||
| 1Third Department of Internal Medicine, Kansai Medical University, 2Department of Gastroenterology, Fujita Health University | |||
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| Background & Aim: HP induces DNA methylation in gastric mucosa, which has been a promising biomarker in gastric cancer (GC). For better understanding of application of HP-related DNA methylation, we examined methylation status of HP-related genes in primary GC and non-neoplastic gastric mucosa in various clinical conditions. Methods: Through genome-wide approach, we chose hyper-methylated genes in HP-positive gastric mucosa (MYOD1, SLC16A12, GDNF, IGF2, MIR 124A1, CDH1, PRDM5, RORA and MLF1). Methylation status of HP-related genes were further examined in 217 primary GC tissues and 635 non-neoplastic gastric mucosa. Results: Adjacent gastric mucosa from GC showed higher methylation Z score of HP-related genes compared to those from cancer-free subjects. In primary GC, higher methylation Z score was correlated with specific subtypes of GC including CpG island methylator phenotype, while lower methylation Z score was associated with aggressive clinicopathological features. We identified magnifying NBI endoscopic feature with hyper-methylated gastric mucosa: demarcated oval/tubulovillous pits with coiled/wavy vessels. Such endoscopic feature presented higher DNA methylation regardless of HP eradication history, and was associated with GC occurrence after HP eradication. Conclusions: HP-related DNA methylation is correlated with GC risk and subtypes of GC, supporting its utility as promising biomarker. Identification of endoscopic feature of hyper methylated gastric mucosa would provide useful information for better clinical implementation. |
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Index Term 1: Helicobacter pylori Index Term 2: DNA methylation |
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